Tris Base CAS 77-86-1 Purity 99.50%~101.0% Biological Buffer Molecular Biology Grade Ultra Pure Factory

Chemical Name: Tris Base Synonyms: Tris(hydroxymethyl)aminomethane  CAS: 77-86-1 Purity: 99.50%~101.0% Appearance: White Crystalline Powder Molecular Biology Grade, Ultra Pure E-Mail: alvin@ruifuchem.com

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Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement. Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of Tris Base (CAS: 77-86-1) with high quality, commercial production. Welcomed to order. Please contact: alvin@ruifuchem.com
Item Specifications
Appearance White Crystalline Powder
Purity 99.50%~101.0%
Melting Point 168.0~171.0℃
Solubility  Clear, Colorless Solution (40% aq. Solution)
Water Content (K.F) ≤0.20%
Insolubles Matter ≤0.005%
Sulphated Ash ≤0.05%
pH  10.0~11.5 (10% aq. solution)
Heavy Metals (as Pb) ≤0.0002%
Iron (Fe) ≤0.0001%
Sulfate (SO4)  ≤0.005%
Chloride (Cl) ≤0.001%
Copper (Cu) ≤0.0001%
Arsenic (As) ≤0.0001%
UV A260nm <0.10 (40% in H2O) 
UV A280nm <0.08 (40% in H2O) 
DNase, RNase, Protease Not Detected
Infrared Spectrum  Conforms to Structure
Test Standard Enterprise Standard
Grade Ultra Pure Grade; Molecular Biology Grade
Usage Biological Buffer; Good's Buffer Component for Biological Research

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Chemical Name Tris Base
Synonyms Tris(hydroxymethyl)aminomethane; Trometamol; Trimethylolaminomethane; 2-Amino-2-(Hydroxymethyl)-1,3-Propanediol; THAM
CAS Number 77-86-1
CAT Number RF-PI1631
Stock Status In Stock, Production Scale Up to Tons
Molecular Formula C4H11NO3
Molecular Weight 121.14  
Solubility Soluble in Water; Insoluble in Chloroform, Ether
Boiling Point 219.0~220.0℃/10 mm Hg(lit.)
Density   1.353 g/cm3
Brand Ruifu Chemical

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Application:

Tris Base (CAS: 77-86-1), or Tris(hydroxymethyl)aminomethane, or known during medical use as Trometamol or THAM. It is extensively used  as a zwitterionic biological buffer in biochemistry and molecular biology research, and enzymatic research. Tris base is widely used as a biological buffer or as a component of buffer formulations such as TAE and TBE buffers. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms (pH 7.0~9.0). Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins. Tris buffer, only nucleic acids and proteins are widely used as solvents.  Tris is used for protein crystal growth under different pH conditions. Tris buffer having a low ionic strength can be used nematode (C. elegans) is formed lamin (lamin) intermediate fibers. Tris is a major component of protein electrophoresis buffer. Tris for the preparation of a surfactant, a vulcanization accelerator, and some drug intermediates.

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