Chemical Properties:
JP16 L-Serine, when dried, contains not less than 98.5% and not more than 101.0% of C3H7NO3. Description L-Serine occurs as white crystals or a crystalline powder. It has a slight sweet taste. It is freely soluble in water and in formic acid, and practically insoluble in ethanol (99.5). It dissolves in 2 mol/L hydrochloric acid TS. Identification Determine the infrared absorption spectrum of L-Serine as directed in the potassium bromide disk method under Infrared Spectrophotometry<2.25>, and compare the spectrum with the Reference Spectrum: both spectra exhibit similar intensities of absorption at the same wave numbers. Optical rotation <2.49> [a]20D: +14.0°~+16.0°(After dry-ing, 2.5 g, 2 mol/L hydrochloric acid TS, 25 mL, 100 mm). pH <2.54> The pH of a solution prepared by dissolving 1.0 g of L-Serine in 10 mL of water is between 5.2 and 6.2. Purity (1) Clarity and color of solution-Dissolve 1.0 g of L-Serine in 10 mL of water: the solution is clear and colorless. (2) Chloride <1.03>-Perform the test with 0.5 g of L-Serine. Prepare the control solution with 0.30 mL of 0.01mol/L hydrochloric acid VS (not more than 0.021%) (3) Sulfate <1.14> Perform the test with 0.6 g of L-Serine. Prepare the control solution with 0.35 mL of 0.005mol/L sulfuric acid VS (not more than 0.028%). (4) Ammonium <1.02>-Perform the test with 0.25 g of L-Serine. Prepare the control solution with 5.0 mL of Standard Ammonium Solution (not more than 0.02%). (5) Heavy Metals <1.07> Proceed with 2.0 g of L-Serine according to Method 1, and perform the test. Prepare the control solution with 2.0 mL of Standard Lead Solution (not more than 10 ppm). (6) Iron <1.10> Prepare the test solution with 1.0 g of L-Serine according to Method 1, and perform the test according to Method A. Prepare the control solution with 1.0mL of Standard Iron Solution (not more than 10 ppm). (7) Related Substances-Dissolve 0.10 g of L-Serine in 10mL of water, and use this solution as the sample solution. Pipet 1 mL of the sample solution, add water to make exactly 10 mL. Pipet 1 mL of this solution, add water to makeexactly 50 mL, and use this solution as the standard solution.Perform the test with these solutions as directed under Thin-layer Chromatography<2.03>. Spot 5mLeachofthesamplesolution and standard solution on a plate of silica gel forthin-layer chromatography. Then develop with a mixture of1-butanol, water and acetic acid (100) (3:1:1) to a distance ofabout 10 cm, and dry the plate at 809C for 30 minutes. Sprayevenly a solution of ninhydrinin a mixture of methanol andacetic acid (100) (97:3) (1 in 100) on the plate, and heat at80℃ for 10 minutes: the spot other than the principal spot obtained from the sample solution is not more intense thanthe spot from the standard solution. Loss on Drying <2.41> Not more than 0.3%(1 g, 105℃, 3 hours). Residue on Ignition <2.44> Not more than 0.1%(1 g). Assay Weigh accurately about 0.11 g of L-Serine, previously dried, dissolve in 3 mL of formic acid, add 50 mL of acetic acid (100), and titrate <2.50> with 0.1 mol/L perchloric acid VS (potentiometric titration). Perform a blank determination in the same manner, and make any necessary correction. Each mL of 0.1 mol/L perchloric acid VS＝10.51 mg of C3H7NO3 Containers and storage Containers-Tight containers. USP39-NF34 DEFINITION L-Serine contains NLT 98.5% and NMT 101.5% of L-serine (C3H7NO3), calculated on the dried basis. IDENTIFICATION A. INFRARED ABSORPTION <197K> ASSAY PROCEDURE Sample: 100mg of Serine Blank: Mix 3mL of formic acid and 50mL of glacial acetic acid. Titrimetric system (See Titrimetry <541>) Mode: Direct titration Titrant: 0.1 N perchloric acid VS Endpoint detection: Potentiometric Analysis: Dissolve the Sample in 3mL of formic acid and 50mL of glacial acetic acid. Titrate with the Titrant. Perform the Blank determination. Calculate the percentage of serine (C3H7NO3) in the sample taken: Result = {[(VS-VB) xNxF]/W} x100 VS= Titrant volume consumed by the Sample (mL) VB= Titrant volume consumed by the Blank (mL) N= actual normality of the Titrant (mEq/mL) F= equivalency factor, 105.1mg/mEq W= Sample weight (mg) Acceptance criteria: 98.5%~101.5% on the dried basis IMPURITIES RESIDUEON IGNITION <281>: NMT 0.1% CHLORIDEAND SULFATE, Chloride <221> Standard solution: 0.50mL of 0.020 N hydrochloric acid Sample: 0.73g of Serine Acceptance criteria: NMT 0.05% CHLORIDEAND SULFATE, Sulfate <221> Standard solution: 0.10mL of 0.020 N sulfuric acid Sample: 0.33g of Serine Acceptance criteria: NMT 0.03% IRON <241>: NMT 30ppm Delete the following: HEAVY METALS, Method I <231>: NMT 15ppm (Official 1-Jan-201) RELATED COMPOUNDS System suitability solution: 0.4mg/mL each of USP L-Serine RS and USP L-Methionine RS in 0.1 N hydrochloric acid Standard solution: 0.05mg/mL of USP L-Serine RS in 0.1 N hydrochloric acid. [NOTE-This solution has a concentration equivalent to about 0.5% of that of the Sample solution.] Sample solution: 10mg/mL of Serine in 0.1 N hydrochloric acid Chromatographic system See Chromatography <621>, Thin-Layer Chromatography.) Mode: TLC Adsorbent: 0.25-mm layer of chromatographic silica gel mixture Application volume: 5μL Developing solvent system: Butyl alcohol, glacial acebutyl alcohol and 2N acetic acid (95:5) System suitability Suitability requirements: The chromatogram of the System suitability solution exhibits two clearly separated spots. Analysis Samples: System suitability solution, Standard solution, and Sample solution. After air-drying the plate, spray with Spray reagent, and heat between 100° and 105° for 15 min. Examine the plate under white light. Acceptance criteria: Any secondary spot of the Sample solution is not larger or more intense than the principal spot of the Standard solution. Individual impurities: NMT 0.5% Total impurities: NMT 2.0% SPECIFIC TESTS OPTICAL ROTATION, Specific Rotation <781S> Sample solution: 100mg/mL in 2N hydrochloric acid Acceptance criteria: +14.0° to +15.6° LOSSON DRYING <731>: Dry a sample at 105℃ for 3 h: it loses NMT 0.2% of its weight. ADDITIONAL REQUIREMENTS ACKAGINGAND STORAGE: Preserve in well-closed containers. USP REFERENCE STANDARDS <11> USP L-Methionine RS USP L-Serine RSShanghai Ruifu Chemical Co., Ltd. is the leading supplier of L-Serine (H-Ser-OH; Abbreviated Ser or S) (CAS: 56-45-1) with high quality. As one of the largest amino acids suppliers in China, Ruifu Chemical supplys amino acids meet AJI, USP, EP, JP and FCC standards. We can provide COA, worldwide delivery, small and bulk quantities available. If you are interested in L-Serine, Please contact: alvin@ruifuchem.comDescription:
JP16 and USP39 Test Methods:
| Chemical Name | L-Serine |
| Synonyms | H-Ser-OH; L-Ser; Abbreviated Ser or S; (-)-Serine; Serine; Laevo-Serine; (S)-Serine; L-2-Amino-3-Hydroxypropionic Acid; (S)-2-Amino-3-Hydroxypropionic Acid; (S)-α-Amino-β-Hydroxypropionic Acid; L-3-Hydroxy-2-Aminopropionic Acid; 3-Hydroxy-L-Alanine; β-Hydroxy-L-Alanine |
| Stock Status | In Stock, Production Capacity 500 Tons per Year |
| CAS Number | 56-45-1 |
| Molecular Formula | C3H7NO3 |
| Molecular Weight | 105.09 |
| Melting Point | 222℃(dec.) (lit.) |
| Density | 1.6 |
| Water Solubility | Soluble in Water, Almost Transparency |
| Solubility | Freely Soluble in Formic Acid. Practically Insoluble in Ethanol and in Ether. Dissolves in Dilute Hydrochloric Acid |
| Storage Temp. | Sealed in Dry, Store at Room Temperature |
| COA & MSDS | Available |
| Classification | Amino Acids & Derivatives |
| Brand | Ruifu Chemical |
Advantages:
How to Purchase? Please contact Dr. Alvin Huang: sales@ruifuchem.com or alvin@ruifuchem.com 15 Years Experience? We have more than 15 years of experience in the manufacture and export of a wide range of high quality pharmaceutical intermediates or fine chemicals. Main Markets? Sell to domestic market, North America, Europe, India, Korea, Japanese, Australia, etc. Advantages? Superior quality, affordable price, professional services and technical support, fast delivery. Quality Assurance? Strict quality control system. Professional equipment for analysis include NMR, LC-MS, GC, HPLC, ICP-MS, UV, IR, OR, K.F, ROI, LOD, MP, Clarity, Solubility, Microbial limit test, etc. Samples? Most products provide free samples for quality evaluation, shipping cost should be paid by customers. Factory Audit? Factory audit welcome. Please make an appointment in advance. MOQ? No MOQ. Small order is acceptable. Delivery Time? If within stock, three days delivery guaranteed. Transportation? By Express (FedEx, DHL), by Air, by Sea. Documents? After sales service: COA, MOA, ROS, MSDS, etc. can be provided. Custom Synthesis? Can provide custom synthesis services to best fit your research needs. Payment Terms? Proforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T (Telex Transfer), PayPal, Western Union, etc.FAQ:
Stable Supply: Maintain reasonable stock
Professional Service: One stop purchasing service
Technical Support: Technology solution available
Custom Synthesis Service: Ranged from grams to kilos
Fast Delivery: If within stock, three days delivery guaranteed
High Quality: Established a complete quality assurance system
Sufficient Capacity: Sufficient facilities and technicians
OEM Package: Custom package and label available
Specifications:
| Items | Inspection Standards | Results |
| Appearance | White Crystals or Crystalline Powder | Conforms |
| Identification | Infrared Absorption Spectrum | Conforms |
| Specific Rotation [α]20/D | +14.4° to +15.5° (C=10, 2N HCl) | -33.8° |
| State of Solution (Transmittance) | Clear and Colorless ≥98.0% | 98.6% |
| Chloride (Cl) | ≤0.020% | <0.020% |
| Sulfate (SO4) | ≤0.020% | <0.020% |
| Ammonium (NH4) | ≤0.020% | <0.020% |
| Iron (Fe) | ≤10ppm | <10ppm |
| Heavy Metals (Pb) | ≤10ppm | <10ppm |
| Arsenic (As2O3) | ≤1.0ppm | <1.0ppm |
| Other Amino Acids | Chromatographically Not Detectable | Conforms |
| Loss on Drying | ≤0.20% (at 105℃, 3 hours) | 0.15% |
| Residue on Ignition (Sulfated) | ≤0.10% | 0.06% |
| Assay | 98.5 to 101.0% (Titration: Dried Basis) | 99.7% |
| pH Test | 5.2 to 6.2 (1.0g in 10ml of H2O) | 5.5 |
| Organic Volatile Impurities | Meets the Requirements | Conforms |
| Origin | Non-Animal Source | Conforms |
| Residual Solvents | Conforms | Conforms |
| Conclusion | Meets AJI97, EP, USP, JP Testing Specifications | |
| Shelf Life | 24 Months From Manufacture Date if Stored Properly | |
| Main Uses | Food Additives; Pharmaceuticals; Nutritional Supplement; etc. | |
63-91-2 - Risk and Safety:
Application:
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S24/25 - Avoid contact with skin and eyes. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| WGK Germany | 3 |
| RTECS | VT8100000 |
| F | 3 |
| TSCA | Yes |
| HS Code | 2922509099 |
Package & Storage:
Package: Fluorinated Bottle, 25kg/bag, 25kg/Cardboard Drum, or according to customer's requirement. Storage Condition: Store in sealed containers at cool, dry and ventilated warehouse away from incompatible substances. Protect from light and moisture.