Chemical Properties:
Package: Fluorinated Bottle, 25kg/bag, 25kg/Cardboard Drum, or according to customer's requirement. Storage Condition: Store in sealed containers at cool, dry and ventilated warehouse away from incompatible substances. Protect from light and moisture.Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of L-Isoleucine (H-Ile-OH) (CAS: 73-32-5) with high quality. As one of the largest amino acids suppliers in China, Ruifu Chemical supplys amino acids and derivatives up to international standards, such as AJI, USP, EP, JP and FCC standard. We can provide COA, worldwide delivery, small and bulk quantities available. If you are interested in L-Isoleucine, Please contact: alvin@ruifuchem.com| Hazard Codes | Xn | RTECS | NR4705000 |
| Risk Statements | 40 | TSCA | Yes |
| Safety Statements | 24/25-36-22 | HS Code | 2922491990 |
| WGK Germany | 3 |
Description:
Safety Information:
Package & Storage:
| Chemical Name | L-Isoleucine |
| Synonyms | H-Ile-OH; L-Ile; L-Ile-OH; L-(+)-Isoleucine; Laevo-Isoleucine; Isoleucine; Abbreviated Ile or I; (2S,3S)-2-Amino-3-Methylpentanoic Acid; (2S,3S)-2-Amino-3-Methylvaleric Acid |
| Stock Status | In Stock, Production Capacity 1500 Tons per Year |
| CAS Number | 73-32-5 |
| Molecular Formula | C6H13NO2 |
| Molecular Weight | 131.18 |
| Melting Point | 284.0~288.0℃(dec.) (lit.) |
| Density | 1.207 g/cm3 (20℃) |
| Water Solubility | Sparing Soluble in Water, 41.2 g/L (50℃) |
| Solubility | Freely Soluble in Formic Acid. Practically Insoluble in Ethanol and in Ether. Dissolves in Dilute Hydrochloric Acid |
| Storage Temp. | Sealed in Dry, Store at Room Temperature |
| COA & MSDS | Available |
| Classification | Amino Acids and Derivatives |
| Brand | Ruifu Chemical |
Advantages:
How to Purchase? Please contact Dr. Alvin Huang: sales@ruifuchem.com or alvin@ruifuchem.com 15 Years Experience? We have more than 15 years of experience in the manufacture and export of a wide range of high quality pharmaceutical intermediates or fine chemicals. Main Markets? Sell to domestic market, North America, Europe, India, Korea, Japanese, Australia, etc. Advantages? Superior quality, affordable price, professional services and technical support, fast delivery. Quality Assurance? Strict quality control system. Professional equipment for analysis include NMR, LC-MS, GC, HPLC, ICP-MS, UV, IR, OR, K.F, ROI, LOD, MP, Clarity, Solubility, Microbial limit test, etc. Samples? Most products provide free samples for quality evaluation, shipping cost should be paid by customers. Factory Audit? Factory audit welcome. Please make an appointment in advance. MOQ? No MOQ. Small order is acceptable. Delivery Time? If within stock, three days delivery guaranteed. Transportation? By Express (FedEx, DHL), by Air, by Sea. Documents? After sales service: COA, MOA, ROS, MSDS, etc. can be provided. Custom Synthesis? Can provide custom synthesis services to best fit your research needs. Payment Terms? Proforma invoice will be sent first after confirmation of order, enclosed our bank information. Payment by T/T (Telex Transfer), PayPal, Western Union, etc.FAQ:
Stable Supply: Maintain reasonable stock
Professional Service: One stop purchasing service
Technical Support: Technology solution available
Custom Synthesis Service: Ranged from grams to kilos
Fast Delivery: If within stock, three days delivery guaranteed
High Quality: Established a complete quality assurance system
Sufficient Capacity: Sufficient facilities and technicians
OEM Package: Custom package and label available
Specifications:
| Items | Inspection Standards | Results |
| Appearance | White Crystals or Crystalline Powder; Slightly Bitter Taste | Conforms |
| Identification | Infrared Absorption Spectrum | Conforms |
| Specific Rotation [α]20/D | +39.5° to +41.5° (C=4, 6N HCl) | +40.2° |
| Transmittanceb | ≥95.0% | 98.3% |
| Chloride (Cl) | ≤0.020% | <0.020% |
| Sulfate (SO4) | ≤0.020% | <0.020% |
| Ammonium (NH4) | ≤0.020% | <0.020% |
| Iron (Fe) | ≤10ppm | <10ppm |
| Heavy Metals (Pb) | ≤10ppm | <10ppm |
| Arsenic (As2O3) | ≤1.0ppm | <1.0ppm |
| Other Amino Acids | Conforms | Conforms |
| Loss on Drying | ≤0.20% | 0.11% |
| Residue on Ignition (Sulfated) | ≤0.10% | 0.06% |
| Assay | 98.5 to 101.0% (Titration: Anhydrous Basis) | 99.7% |
| pH Test | 5.5 to 6.5 (0.5g in 20ml of H2O) | 5.8 |
| Origin | Non-Animal Source | Conforms |
| Residual Solvents | Conforms | Conforms |
| Conclusion | This Product by Inspection Accords with the Standard of AJI97; USP | |
| Main Uses | Amino Acids; Food Additives; Pharmaceutical Intermediates | |
Application:
L-Isoleucine (H-Ile-OH) (CAS: 73-32-5) Usage L-Isoleucine, also known as Isoleucine, is an amino acid that is an isomer of leucine. It is important in hemoglobin synthesis and regulation of blood sugar and energy levels. Used for amino acid injection, compound amino acid infusion, food additives. L-Isoleucine is one of the essential amino acids that cannot be made by the body and is known for its ability to help endurance and assist in the repair and rebuilding of muscle. This amino acid is important to body builders as it helps boost energy and helps the body recover from training. L-Isoleucine is also classified as a branched-chain amino acid (BCAA). There are three branched-chain amino acids in the body, with the others being L-Valine, and L-Leucine, and all of them help promote muscle recovery after exercise. Isoleucine is actually broken down for energy within the muscle tissue. Function: 1. L-Isoleucine provides ingredients for the manufacturing of other essential biochemical components in the body, some of which are utilized for the production of energy, stimulants to the upper brain and helping you to be more alert. 2. L-Isoleucine can repair muscle,control blood sugar and provide energy for body tissue together with isoleucine and valine. It can improve the production of growth hormone and help burning visceral fat because it can resolve into glucose rapidly. Function and Application: 1. Food grade L-Isoleucine is used for all kinds of amino acid nutraceuticals, sports and fitness nourishment, amino acid functional beverage. And as an important the food additive, used to strengthen all kinds of food, and improve the nutrition value of food. 2. Pharmaceutical grade L-Isoleucine is as an amino acid fluid infusion, can replace sugar metabolism and provide energy, it is more precious amino acid API, treatment of special amino acid type of drugs such as liver, and liver spirit liquid.73-32-5 AJI 97 Test Method:
L-Isoleucine (H-Ile-OH) (CAS: 73-32-5) AJI 97 Test Method Identification: Compare the infrared absorption spectrum of the sample with that of the standard by potassium bromide disc method. Specific Rotation [α]20/D: Dried Sample, C=4, 6mol/L HCl State of Solution (Transmittance): 0.5g in 10ml of 1mol/L, HCl spectrophotometer, 430nm, 10nm cell thickness. Chloride (Cl): 0.7g, A-1, ref: 0.40ml of 0.01mol/L HCl Sulfate (SO4): 1.2g, (1), ref: 0.50ml of 0.005mol/L H2SO4 Iron (Fe): 1.5g, (1), ref: 1.5ml of Iron Std. (0.01mg/ml) Heavy Metals (Pb): 2.0g, (1), dissolve by warming, ref: 2.0ml of Pb Std. (0.01mg/ml) Arsenic (As2O3): 2.0g, (1), ref: 2.0ml of As2O3 Std. Other Amino Acids: Test Sample: 50μg, B-6-a, control: L-Ile 0.25μg Loss on Drying: at 105℃ for 3 hours. Assay: Dried sample, 130mg, (3), 3ml of formic acid, 50ml of glacial acetic acid, 0.1mol/L HCLO4 1ml=13.117mg C6H13NO2 pH Test: 0.5g in 20ml of H2O L-Isoleucine (H-Ile-OH) (CAS: 73-32-5) USP35 Test Method DEFINITION Isoleucine contains NLT 98.5% and NMT 101.5% of L-Isoleucine (C6H13NO2), calculated on the dried basis. IDENTIFICATION A. INFRARED ABSORPTION <197K> ASSAY PROCEDURE Sample: 130 mg of Isoleucine Blank: Mix 3 mL of formic acid and 50 mL of glacial acetic acid. Titrimetric system (See Titrimetry <541>) Mode: Direct titration Titrant: 0.1 N perchloric acid VS Endpoint detection: Potentiometric Analysis: Dissolve the Sample in 3 mL of formic acid and 50mL of glacial acetic acid. Titrate with the Titrant. Perform the Blank determination. Calculate the percentage of Isoleucine (C 6H13NO2) in the Sample taken: Result = {[(VS-VB) x N x F]/W} x100 VS= Titrant volume consumed by the Sample (mL) VB= Titrant volume consumed by the Blank (mL) N= actual normality of the Titrant (mEq/mL) F= equivalency factor, 131.2 mg/mEq W= Sample weight (mg) Acceptance criteria: 98.5%~101.5% on the dried basis IMPURITIES RESIDUEON IGNITION <281>: NMT 0.3% CHLORIDE AND SULFATE, Chloride <221> Standard solution: 0.50mL of 0.020 N hydrochloric acid Sample: 0.73g of Isoleucine Acceptance criteria: NMT 0.05% CHLORIDE AND SULFATE, Sulfate <221> Standard solution: 0.10mL of 0.020 N sulfuric acid Sample: 0.33g of Isoleucine Acceptance criteria: NMT 0.03% IRON <241>: NMT 30ppm Delete the following: HEAVY METALS, Method I <231>: NMT 15ppm RELATED COMPOUNDS System suitability solution: 0.4mg/mL each of USP L-Isoleucine RS and USP L-Valine RS in 0.1 N hydrochloric acid Standard solution: 0.05mg/mL of USP L-Isoleucine RS in 0.1 N hydrochloric acid. [NOTE-This solution has a concentration equivalent to 0.5% of that of the Sample solution.] Sample solution: 10mg/mL of Isoleucine in 0.1 N hydrochloric acid Chromatographic system (See Chromatography <621>, Thin-Layer Chromatography.) Mode: TLC Adsorbent: 0.25-mm layer of chromatographic silica gel mixture Application volume: 5μL Developing solvent system: Butyl alcohol, glacial acetic acid, and water (3:1:1) Spray reagent: 2 mg/mL of ninhydrin in a mixture of butyl alcohol and 2N acetic acid (95:5) System suitability Suitability requirements: The chromatogram of the System suitability solution exhibits two clearly separated spots. Analysis Samples: System suitability solution, Standard solution, and Sample solution. After air-drying the plate, spray with Spray reagent, and heat between 100° and 105° for 15 min. Examine the plate under white light. Acceptance criteria: Any secondary spot of the Sample solution is not larger or more intense than the principal spot of the Standard solution. Individual impurities: NMT 0.5% Total impurities: NMT 2.0% SPECIFIC TESTS OPTICAL ROTATION, Specific Rotation <781S> Sample solution: 40 mg/mL in 6 N hydrochloric acid Acceptance criteria: +38.9° to +41.8° PH <791> Sample solution: 10 mg/mL solution Acceptance criteria: 5.5~7.0 LOSSON ON DRYING <731>: Dry a sample at 105℃ for 3 h: it loses NMT 0.3% of its weight. ADDITIONAL REQUIREMENTS PACKAGING AND STORAGE: Preserve in well-closed containers. USP REFERENCE STANDARDS <11> USP L-Isoleucine RS USP L-Valine RS