Chemical Properties:
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement. Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.Manufacturer Supply With High Quality, Commercial Production Chemical Name: Thiazolyl Blue Tetrazolium Bromide (MTT) CAS: 288-47-1| Item | Specifications |
| Appearance | Yellow to Dark Yellow Powder |
| Purity / Analysis Method | >99.0% (Titration With AgNO3) |
| Melting Point | 195℃ (dec.) (lit.) |
| Solubility in Hot Water | Clear |
| Loss on Drying | <1.00% |
| Total Impurities | <1.00% |
| Colorimetric Test | Turn Red Purple With 35ppm D-Glucose |
| Test Standard | Enterprise Standard |
| Usage | Colorimetric Agent; Used in Measurement of Cell Proliferation |
Description:
Specifications:
Package & Storage:
| Chemical Name | Thiazolyl Blue Tetrazolium Bromide |
| Synonyms | MTT; Thiazolyl Blue; 3-(4,5-Dimethyl-2-Thiazolyl)-2,5-Diphenyltetrazolium Bromide |
| CAS Number | 298-93-1 |
| CAT Number | RF-PI1147 |
| Stock Status | In Stock, Production Scale Up to Tons |
| Molecular Formula | C18H16BrN5S |
| Molecular Weight | 414.33 |
| Brand | Ruifu Chemical |
Advantages:
FAQ:
Application:
Thiazolyl Blue Tetrazolium Bromide (MTT) (CAS: 298-93-1) is a colorimetric agent widely used to measure cell proliferation. MTT is reduced from yellow color to purple formazan in living cells. MTT is a common dye used in Cell Proliferation assays or Cell Growth assays. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
