Chemical Properties:
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement. Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of HEPES Sodium Salt (CAS: 75277-39-3) with high quality, commercial production. Welcome to order. Please contact: alvin@ruifuchem.com| Item | Specifications |
| Grade | Ultra Pure Grade |
| Appearance | White Powder |
| Purity / Analysis Method | >99.5% (Titration, Dried basis) |
| Loss on Drying | <3.00% |
| Residue on Ignition | <0.20% |
| Heavy Metals (as Pb) | <0.0005% |
| Sulphate (SO4) | <0.01% |
| Iron (Fe) | <0.0005% |
| UV (1M in H2O) A260nm | <0.20 |
| UV (1M in H2O) A280nm | <0.20 |
| UV (1M in H2O) A440nm | <0.20 |
| Solubility (0.1M, Water) | Clear Colorless Solution |
| pH | 9.5~10.5 (10% in Water) |
| pKa (25℃) | 7.35~7.75 |
| DNase Activity | Not Detected |
| RNase Activity | Not Detected |
| Protease Activity | Not Detected |
| Infrared Spectrum | Conforms to Structure |
| Test Standard | Enterprise Standard |
| Usage | Biological Buffer |
Description:
Specifications:
Package & Storage:
| Chemical Name | HEPES Sodium Salt |
| Synonyms | HEPES-Na; 2-[4-(2-Hydroxyethyl)-1-Piperazinyl] ethanesulfonic Acid Sodium Salt; 4-(2-Hydroxyethyl)-1-Piperazineethanesulfonic Acid Sodium Salt |
| CAS Number | 75277-39-3 |
| CAT Number | RF-PI1656 |
| Stock Status | In Stock |
| Molecular Formula | C8H17N2O4SNa |
| Molecular Weight | 260.30 |
| Melting Point | 234℃ |
| Brand | Ruifu Chemical |
Advantages:
FAQ:
Application:
HEPES Sodium Salt Solution (CAS: 75277-39-3) is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. HEPES Sodium Salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8~8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris.
