Chemical Properties:
Package: Bottle, Aluminium foil bag, 25kg/Cardboard Drum, or according to customer's requirement. Storage Condition: Store in sealed containers at cool and dry place; Protect from light and moisture.Shanghai Ruifu Chemical Co., Ltd. is the leading manufacturer and supplier of AMPSO (CAS: 68399-79-1) with high quality, commercial production. Welcome to order. Please contact: alvin@ruifuchem.comItem | Specifications |
Appearance | White Crystalline Powder |
Purity / Analysis Method | >99.0% (Titration with NaOH) |
Useful pH Range | 8.3~9.7 |
pKa (25℃) | 9.0 |
Melting Point | 215.0~225.0℃ |
Loss on Drying | <1.00% |
Sulphated Ash | <0.10% |
Heavy Metals (as Pb) | <0.0005% |
Chloride (Cl) | <0.02% |
Sulphate (SO4) | <0.05% |
Iron (Fe) | <0.0005% |
OD260nm | <0.10 (0.1M aq Solution) |
OD280nm | <0.10 (0.1M aq Solution) |
Solubility in Water | Clear to Slightly Hazy Colorless Solution (10% aq.) |
Test Standard | Enterprise Standard |
Usage | Biological Buffer |
Description:
Specifications:
Package & Storage:
Chemical Name | AMPSO |
Synonyms | AMPSO, Free Acid; N-(1,1-Dimethyl-2-Hydroxyethyl)-3-Amino-2-Hydroxypropanesulfonic Acid; 2-Hydroxy-3-((1-Hydroxy-2-Methylpropan-2-yl)amino)propane-1-Sulfonic Acid; 3-((1,1-Dimethyl-2-Hydroxyethyl)amino)-2-Hydroxypropanesulfonic Acid |
CAS Number | 68399-79-1 |
CAT Number | RF-PI1682 |
Stock Status | In Stock, Production Scale Up to Tons |
Molecular Formula | C7H17NO5S |
Molecular Weight | 227.28 |
Brand | Ruifu Chemical |
Advantages:
FAQ:
Application:
AMPSO (CAS: 68399-79-1) is an alkaline transfer buffer which facilitates nearly complete transfer of strongly basic proteins from gels to nitrocellulose without lowering the transfer efficiency for other proteins. The most common buffer for the blotting of proteins to nitrocellulose is the Tris-Glycine buffer (pH 8.3), however the transfer of strongly basic proteins can be very poor (~20%). An alkaline transfer buffer, such as 25 mM AMPSO, pH 9.5 allows for almost complete transfer of strongly basic proteins from gels to nitrocellulose without lowering the transfer efficiency for other proteins. AMPSO buffer is also used in PCR amplification and may be useful in several additional applications.